How to use MEGA software for evolutionary studies of HSP

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Molecular Evolutionary Genetics Analysis (MEGA) is computer software for conducting statistical analysis of molecular evolution and for constructing phylogenetic trees.The video about how to perform evolutionary genetic analysis of heat shock protein and comparative analysis using MEGA software.Presented about inducing thermotolerance in banana by induction of hsp gene from ideal model sequence. HSPgene has major role in control of heat stress. Here Brassica juncea species and Musa acuminata species have more similarity when compared to other species since this is taken as template gene for thermotolerance induction.To compare the similarity i compute the substitution matrix ,pairwise distances,phylogeny tree construction,find gene duplications and find evolutionary probability among that 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