Youtor
Easy SDS PAGE Gel Electrophoresis Protocol and Theory
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=mabx-U5X9J8
Learn SDS PAGE in 30 minutes with this deep-dive tutorial into the SDS PAGE Gel Electrophoresis Protocol and a SDS PAGE Theory. • SDS-Page stands for sodium dodecyl surface polyarcylamide gel electrophoresis, which is a technique to separate proteins using an electric field. In this tutorial we utilize a protocol from rockland antibodies (https://rockland-inc.com/sds-page.aspx) to demonstrate every step and to whiteboard why we do individual tasks within the protocol. • Things we cover include: • What is SDS Page • Why do you use polyacrylamide gels vs. agarose gels • What is porosity and how does it affect protein separation • Why do you need running buffer • What roles do Beta mercapto ethanol and SDS plan in the procedure • How do you assemble the gel running apparatus • Why do you add a sample running dye to samples • How do you run a gel • What purpose does a protein ladder play • How do you know when SDS Page has been completed • What follow on analyses can you do with Coomassie or Western Blotting • You should also visit our website http://www.scigine.com (a search engine for biology and biochemistry methods) which provides free protocols for specific targets that you might want to run on SDS Page. • Here's a specific western blotting protocol that you might like from our website (http://www.scigine.com/blog/western-b...) • Here are other specific SDS Page protocols: • http://scigine.com/method.php?ID=1897 • http://scigine.com/method.php?ID=1842
#############################
