Multiple Gene Knockout Mouse Small Intestinal Organoids Using CRISPRConcatemer l Protocol Preview

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=nKLS5xVGAzk

Watch the Full Video at https://www.jove.com/v/55916/a-protoc.... • A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer - a 2 minute Preview of the Experimental Protocol • Alessandra Merenda, Amanda Andersson-Rolf, Roxana C. Mustata, Taibo Li, Hyunki Kim, Bon-Kyoung Koo • University of Cambridge, Wellcome Trust - Medical Research Council Stem Cell Institute; University of Cambridge, Department of Genetics; Yonsei University College of Medicine, Department of Pathology; • This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this method. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments

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