Counting Cells with a Hemocytometer











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There can be tens of thousands of cells in one milliliter of culture medium. So how are cells counted? • The process requires diluting the cell culture, dying the cells, and loading the cells into a hemocytometer. A hemocytometer is a modified microscope slide with two chambers. Each chamber is divided into 9 squares. There are specific guidelines for counting cells within the squares. • In this video, we'll demonstrate the process of counting cells using a hemocytometer. We'll also work through some of the typical calculations that accompany cell counting. • Our hope is that after viewing this video, you will feel more confident and be better prepared to try counting cells in a hands-on lab experience. • Practice using our FREE virtual hemocytometer: https://www.ncbionetwork.org/educatio... • This video was filmed on location at Rowan-Cabarrus Community College.

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