Fluorescence from eggshells protoporphyrin IX

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Fluorescence from eggshells • Liberation of Protoporphyrin IX by acidic dissolution of eggshell cuticle • Fresh brown hen eggs (from Gallus domesticus) show a red fluorescence under UV-light, caused by the enclosed porphyrines in the cuticle of the egg shell that are reponsible for their brown color. By dissolving the membrane-free carbonat layer the porphyrins are released from the cuticle, which only make up the outermost 3% of the shell, and dissolve in the unpolar phase (ethyl acetate) of the solvent system (aqueous hydrochloric acid - ethyl acetate). Protoporphyrin IX is a photosensitive compound that is found as its iron complex in haemoglobin and is related to the chlorophylls that also show a red fluorescence in solution when excited at 365 nm. Other porphyrins found in eggshells are coproporphyrin and uroporphyrin. • Equipment: • fresh brown hen egg, without eggshell membrane • UV-light source (366 nm) • hydrochlorid acid, ethyl acetate • Canon EOS 500D, LAOWA 60 mm • Software: • Sony Vegas Pro 13.0 • Adobe Photoshop Lightroom 5.0 • Spartan '14 • Chimera 1.11.2 • ChemDraw 12 • Music: • Kai Engel - modum (edited) • http://freemusicarchive.org/music/Kai... • References: • D. Weiß, H. Brandl, Chemie in unserer Zeit, 47, 122-131 • https://doi.org/10.1002/ciuz.201300618 • M.R. Lang, J.W. Wells, World's Poultry Science Journal 1987, 43(3), 238-246. • https://doi.org/10.1079/WPS19870016 • G.F. Stewart, Poult Sci. 1935 14(1), 24-32. • https://doi.org/10.3382/ps.0140024 • T.K. With, Biochemical Journal 1974, 137(3), 596.2-598. • https://www.ncbi.nlm.nih.gov/pmc/arti... • Deoxy Human Haemoglobin 1A3N (from RCSB PDB) • https://www.rcsb.org/pdb/explore/expl...

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