Episode 011 CRISPR Genome Editing Basics

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Life and Biology - Episode 11 - CRISPR: Genome Editing Basics • Dr. Joel Graff • In this video, I introduce genome editing and focus on how the CRISPR-Cas system can be used to precisely cut a genome in the • correct location. • Relevant links: • A single guide RNA can be formed with a linker connecting the crRNA and the tracrRNA: • http://www.sciencemag.org/content/337... (Charpentier/Doudna labs) • Proof of concept: Genome editing in human cells: • http://cdn.elifesciences.org/elife-ar... (Doudna lab) • http://www.sciencemag.org/content/339... (Church lab) • http://www.sciencemag.org/content/339... (Zhang lab)

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