Labster Virtual Lab Microscopy Simulation

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=s3odnxw9uHI

Get access here: https://www.labster.com/simulations/m... • ABOUT THE MICROSCOPY VIRTUAL LAB SIMULATION • In the Microscopy lab, students examine chicken intestinal sections using three different microscopy techniques: light microscope, transmission electron microscope and fluorescence microscope. Students are presented with a research project investigating intestinal—similar to coeliac disease. • SUMMARIZED LEARNING OUTCOMES • Understand different microscopy techniques and their limitations • Identify various cell types and cellular structures • Learn about coeliac disease and intestinal inflammation • Learn about staining techniques • DETAILED LAB DESCRIPTION • Light microscopy • Students learn how to operate a light microscope and understand the mechanisms behind. They are presented with chicken intestinal slides that have been stained with Anilin, Orange G and Fuchsin. Using the 5x magnification, students identify the villus, and then proceed with higher magnifications to identify smooth muscle, extra-cellular tissue, epithelial cells, Goblet cells and the nuclei. • Transmission electron microscopy • Electron microscopes can be used to visualize objects that are too small to see when using a light microscope—for example the micro-villi, mitochondria and the junctions between cells. In this lab, students examine a chicken intestine slide that is specially prepared for a transmission electron microscope. Students can zoom in and out and observe different cellular structures. • Fluorescence microscopy • Students learn about fluorescence staining techniques and how it can be used to visualize specific structures. For example, by staining the DNA with DAPI, students can easily identify a cell’s nucleus. In this part of the lab, Students will examine a chicken intestine sample that is infected with a retrovirus. They observe how the virus infects the lymphocytes and how it inhibits inflammation. The retrovirus can be further developed as medicine for coeliac disease.

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