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ProtParam Tool of ExPASy Protein Analysis Bioinformatics Basic Science Series
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ProtParam Tool of ExPASy | Protein Analysis | Bioinformatics | Basic Science Series • Keywords: ProtParam tool ExPASy, Protein analysis bioinformatics, ExPASy protein sequence, Molecular weight protein, Theoretical pI protein, Amino acid composition tool, Atomic composition protein, Protein extinction coefficient, Estimated protein half-life, Protein instability index, Aliphatic index protein, GRAVY protein analysis, Protein physico-chemical properties, Protein characterization tool, Protein structure-function relationship, Protein drug design, Protein stability analysis, Protein biotechnology applications, Functional annotation protein, Comparative protein studies, Protein engineering bioinformatics, Protein sequence FASTA format, Protein molecular biology, Enzyme kinetics protein, Protein-protein interactions analysis, Genetic engineering protein stability, Recombinant protein expression, Bioinformatics protein tools, ExPASy server protein analysis, Comprehensive protein analysis, Protein research tools • The ProtParam tool, available on the ExPASy (Expert Protein Analysis System) server, is a powerful resource for the computational analysis of protein sequences. It provides a range of physico-chemical properties that are crucial for understanding protein functions and characteristics. Here's a detailed overview of ProtParam, its features, and its significance in protein research. • Overview of ProtParam • ProtParam analyzes a given protein sequence and computes various properties, including: • 1. Molecular Weight: Determines the molecular mass of the protein. • 2. Theoretical pI (Isoelectric Point): Calculates the pH at which the protein has no net charge. • 3. Amino Acid Composition: Provides the frequency of each amino acid in the protein sequence. • 4. Atomic Composition: Lists the number of atoms of carbon, hydrogen, nitrogen, oxygen, and sulfur. • 5. Extinction Coefficient: Estimates how much light a protein absorbs at a specific wavelength, useful for concentration determination. • 6. Estimated Half-Life: Predicts the stability of the protein in a given environment. • 7. Instability Index: Classifies the protein as stable or unstable. • 8. Aliphatic Index: Relates to the volume occupied by aliphatic side chains, indicating the protein's thermal stability. • 9. Grand Average of Hydropathicity (GRAVY): Reflects the overall hydrophobic or hydrophilic nature of the protein. • Applications and Benefits • Research and Development: • Protein Characterization: Essential for understanding protein structure-function relationships. • Drug Design: Helps in identifying potential drug targets by analyzing protein stability and behavior. • Biotechnology: Assists in engineering proteins with desired properties for industrial applications. • Academic Research: • Functional Annotation: Provides insights into the possible functions of newly discovered proteins. • Comparative Studies: Allows comparison of protein properties across different species or conditions. • Protein Engineering: Guides modifications to improve protein stability or activity. • These bullet points highlight the main aspects of each field, their scope, practical examples, and job opportunities. • WhatsApp group: https://chat.whatsapp.com/HTXQC1Ax2qf... • Twitter: / basic_series • Facebook Group: / 742235929758671 • LinkedIn: / basicscienceseriesa54439208 • Instagram: / basic_science_series • Support my work at https://www.patreon.com/user?u=37177596 • Disclaimer: The information provided is for educational purposes only. The content of this channel should not be considered as medical advice of any kind. Use this information at your own risk. We hold no responsibility for any issue, concerns, or damage arising from the content of the video. Under no circumstances this channel be responsible or liable in any way for any content, including but not limited to, any errors or omissions in the content, any loss, any damage of any kind incurred as a result of any content communicated in this video, whether by this channel or a third party. In no event shall this channel be liable for any special indirect or consequential damages of any damages whatsoever resulting from the content of the channel.
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