SplitUbiquitin Based MYTH System Identifying ProteinProtein Interactions l Protocol Preview











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Watch the Full Video at https://www.jove.com/v/1698/split-ubi.... • Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions - a 2 minute Preview of the Experimental Protocol • Jamie Snider, Saranya Kittanakom, Jasna Curak, Igor Stagljar • University of Toronto, Department of Biochemistry; University of Toronto, Department of Molecular Genetics; University of Toronto, Terrence Donnelly Centre for Cellular and Biomolecular Research (CCBR); • MYTH allows the sensitive detection of transient and stable interactions between proteins that are expressed in the model organism Saccharomyces cerevisiae. It has been successfully applied to study exogenous and yeast integral membrane proteins in order to identify their interacting partners in a high throughput manner. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel:    / jovejournalofvisualizedexperiments  

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