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Complete Guide to qRTPCR StepbyStep Protocol96well plateDNARNAPolymerase Chain reaction
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=ykr1ho1fm7E
🔬 Complete Guide to qRT-PCR: Step-by-Step Protocol 🔬 • Welcome to our latest video, where we provide a comprehensive step-by-step guide on quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR)! 🧬 Whether you're a beginner in molecular biology or looking to refine your skills, this video is designed for you. • In this video, we’ll walk you through the entire qRT-PCR process, from RNA extraction to data analysis. You’ll gain insights into why we use housekeeping genes and internal controls, ensuring accurate and reliable results. • 📚 What You’ll Learn: • RNA Extraction: The crucial first step to ensure high-quality RNA for your experiments. • Preparation of Reaction Mix: Learn how to create the perfect mix for optimal PCR results. • Preparation of 96-Well Plate: Step-by-step instructions on how to set up your 96-well plate for the qRT-PCR. • qRT-PCR Setup: An overview of the equipment and settings needed for your qRT-PCR run. • Loading Plates: Tips on how to properly load your samples into the plate for accurate readings. • Running qPCR: Understanding the process of running the qPCR in our lab. • Data Analysis: Interpreting the results and what the data means for your research. • We will be conducting the qRT-PCR in our lab, allowing you to observe each step in action! 📊 This video serves as a complete guide for those new to molecular biology and looking to learn the essential techniques of qRT-PCR. • • #qRTPCR #MolecularBiology #RNAExtraction #PCRSetup #DataAnalysis #HousekeepingGene #InternalControl #LabTechniques #ScienceEducation #DrSajjadAsaf #BiologyStudents #Research
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