RNA purification

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=yzqhPBAvvY0

For more information, log on to- • http://shomusbiology.weebly.com/ • Download the study materials here- • http://shomusbiology.weebly.com/bio-m... • Source of all articles published in description is Wikipedia. Thanks to original content developers. • Link- http://en.wikipedia.org/wiki/Main_Page • This video describes the purification process of mRNA using oligo dT column. • Polyadenylation is the covalent linkage of a polyadenylyl moiety to a messenger RNA molecule. In eukaryotic organisms, with the exception of histones, all messenger RNA (mRNA) molecules are polyadenylated at the 3' end. The poly(A) tail and the protein bound to it aid in protecting mRNA from degradation by exonucleases. Polyadenylation is also important for transcription termination, export of the mRNA from the nucleus, and translation. mRNA can also be polyadenylated in prokaryotic organisms, where poly(A) tails act to facilitate, rather than impede, exonucleolytic degradation. • Polyadenylation occurs during and immediately after transcription of DNA into RNA. After transcription has been terminated, the mRNA chain is cleaved through the action of an endonuclease complex associated with RNA polymerase. After the mRNA has been cleaved, around 250 adenosine residues are added to the free 3' end at the cleavage site. This reaction is catalyzed by polyadenylate polymerase. Just as in alternative splicing, there can be more than one polyadenylation variant of an mRNA.

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