youtor.org
Expression screening genomic library screening
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Video Source: www.youtube.com/watch?v=EuQjcveo0Nk
Lecture explains the process of genomic library screening after the cloning is done. http://shomusbiology.weebly.com/ • Download the study materials here- • http://shomusbiology.weebly.com/bio-m... • A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using the enzyme, DNA ligase.[1] Next, the vector DNA can be taken up by a host organism- commonly a population of Escherichia coli or yeast- with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.[2] • There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.[3] • Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.[4] Genomic libraries are also utilized in comparison studies between differing species. • In order to isolate clones that contain regions of interest from a library, the library must first be screened. One method of screening is hybridization. Each transformed host cell of a library will contain only one vector with one insert of DNA. The whole library can be plated onto a filter over media. The filter and colonies are prepared for hybridization and then labeled with a probe.[13] The target DNA- insert of interest- can be identified by detection such as autoradiography because of the hybridization with the probe as seen below. Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia. • Link- http://en.wikipedia.org/wiki/Main_Page
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