Practical quotFormation of osazone from glucose quot

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=SKE2B7Vfykg

This is the video on osazone test for carbohydrates with demonstration and discussion of viva questions. • It contains • 00:00 - Introduction • 00:24 - Osazone Formation • 00:51 - Principle of Osazone Test • 02:06 - Osazone Test Procedure • 02:57 - Osazone crystals of various sugar • 04:14 - Discussion of viva questions • 05:13 - Demonstration of Osazone Test • Osazone Formation • All reducing sugars will form osazones with excess of phenylhydrazine when kept at boiling temperature. • Osazones are insoluble. • Each sugar will have characteristic crystal form of osazones. • Principle • Osazone formation results in the formation of characteristic crystalline derivatives of monosaccharides or disaccharides. • In this reaction, phenylhydrazine will react with carbonyl carbon (aldehyde or ketone group) and the next adjacent carbon of monosaccharides or reducing disaccharides • Subscribe my channel from - / biochemistrybasicsbydramit • For more interesting stuffs related to biochemistry visit - • https://www.biochemistrybasics.com/ • Follow me • On Twitter - / dr_biochemistry • On Instagram - / biochemisrybasics • On Facebook - / amit.v.m • #osazones #osazonetest #biochemistry

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