RobotHighThroughput Crystallization Membrane Proteins In Lipidic Mesophases l Protocol Preview

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Watch the Full Video at https://www.jove.com/v/4000/use-robot.... • Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases - a 2 minute Preview of the Experimental Protocol • Dianfan Li, Coilín Boland, Kilian Walsh, Martin Caffrey • Trinity College Dublin , Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry Immunology; • Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments

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