SEAonaCHIP device functioning 3D video

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SEA-on-a-CHIP: Real time monitoring of SEA contaminants by an autonomous Lab-on-a-CHIP biosensor • The SEA-on-a-CHIP device is an autonomous, miniaturized on site analytical system for real-time monitoring of harmful contaminants in seawater, developed to be used as an early warning system in aquaculture facilities. • The floating watertight outer casing conceals a highly-engineered sampling and micro-fluidic unit which manages the whole analytical procedure. This is associated with a communication module for data transmission to a land or sea-based receiver. • The battery pack and liquid reservoirs allow the device to work autonomously for one month affording two daily seawater samplings and simultaneous analyses of 7 pollutants, and one control. • First, the sampling system pumps the seawater from 1 meter depth into a solid phase extraction column to concentrate the contaminants. • 500 mL of seawater passes through the column where the contaminants are retained within an adsorbent cartridge. The clean water is eliminated through the output pipe. . • Next, an elution solvent is passed through the adsorbant cartridge to elute the concentrated target pollutants. • Then, tThe resulting concentrated solution is transported through the microfluidic system into a dilution tank to render the solution compatible with the subsequent bioassays. • Here, the sample is also exposed to known amounts of 7 antibodies, each one specific for the 7 target pollutants. • Each antibody combines with its target compound to form pollutant-antibody complexes. • A few millilitres of the resulting solution is pumped into each of the 8 parallel biosensors for simultaneous indirect competitive immunoassays. • The remaining free antibodies bind with their complementary antigens immobilized onto the respective microelectrode surfaces. • This binding causes a signal that is proportional to the quantity of pollutant molecules contained in the sample. The signal is measured by amperometry. • An electronic board filters and amplifies the signals from the biosensors. Finally, the On Mote Host System transmits the data via a ZigBee protocol connection to a monitoring gateway placed on land or at sea which receives the signals relating to the deployment of the sensors. • A MoGa sends all data to the cloud for storage, so the aquaculture manager can check and manage the device in real time from his/her computer. • For more information visit the website: 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