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Introduction to Bioluminescent Assays including the chemistry of bioluminescence
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Video Source: www.youtube.com/watch?v=h33ygtGsoz8
This video provides an introduction to bioluminescent assays, including the chemistry of bioluminescence and the three assays. • The conventional use of reporter genes is largely to analyse gene expression and dissect the function of cis-acting genetic elements such as promoters and enhancers (so-called promoter bashing ). • In typical experiments, deletions or mutations are made in a promoter region, and their effects on coupled expression of a reporter gene are quantitated. However, reporter genes also can be used to study other cellular events, including events that are not related to gene expression such as cell health and signaling pathways. • For more information about reporter gene applications after you have watched the video go to https://www.promega.co.uk/resources/g...
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