Tissue Culture Series 1 How to Thaw Cells with High Efficiency
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Video Source: www.youtube.com/watch?v=nSd9BfMD6u4
To learn more, visit http://ms.spr.ly/6059w1WWz • Tutorial demonstrating best practices for starting a fresh cell culture from frozen stocks, including cell thawing tips and tricks to maximize survival. • • Ice crystals and thaw time • • How to avoid osmotic shock • • Cryoprotectant removal and media replacement • • Thawing stem cells and primary cells • • Methods for resuspending cells • • Seeding density considerations • • Troubleshooting
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