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Multiplex PCR
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For more information, log on to- • http://shomusbiology.weebly.com/ • Download the study materials here- • http://shomusbiology.weebly.com/bio-m... • Multiplex polymerase chain reaction (Multiplex PCR) is a modification of polymerase chain reaction in order to rapidly detect deletions or duplications in a large gene. This process amplifies genomic DNA samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler. Multiplex-PCR was first described in 1988 as a method to detect deletions in the dystrophin gene.[1] It has also been used with the steroid sulfatase gene.[2] In 2008, multiplex-PCR was used for analysis of microsatellites and SNPs.[3] • Multiplex-PCR consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes, i.e., their base pair length, should be different enough to form distinct bands when visualized by gel electrophoresis. Commercial multiplexing kits for PCR are available and used by many forensic laboratories to amplify degraded DNA samples. Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia. • Link- http://en.wikipedia.org/wiki/Main_Page
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