Yeast Luminometric amp Xenopus Oocyte Electrophysiological ExaminationsTRPV4 l Protocol Preview

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Watch the Full Video at https://www.jove.com/v/50816/yeast-lu.... • Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4 - a 2 minute Preview of the Experimental Protocol • Jinfeng Teng, Stephen Loukin, Xinliang Zhou, Ching Kung • University of Wisconsin – Madison, Laboratory of Cell and Molecular Biology; University of Wisconsin – Madison, Department of Genetics; • To complement commonly used methods to study TRPV4’s, two methods are described: Its mechanosensitivity can be studied by Ca2+-aequorin luminometry in transgenic yeast upon hypo-osmotic challenge. It can also be examined in TRPV4-RNA injected Xenopus oocytes by whole-cell two-electrode voltage clamp or patch clamp in on-cell or excised mode. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments

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