Apoptosis in Hypothalamic Cell Model by Caspase Multiplexing Assay Protocol Preview

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Watch the Full Video at https://www.jove.com/v/51305/use-casp.... • Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model - a 2 minute Preview of the Experimental Protocol • Tammy A. Butterick, Cayla M. Duffy, Rachel E. Lee, Charles J. Billington, Catherine M. Kotz, Joshua P. Nixon • Minneapolis Veterans Affairs Health Care System, Department of Veterans Affairs; University of Minnesota, Department of Food Science and Nutrition; University of Minnesota, Department of Integrative Biology and Physiology; University of Minnesota, Department of Medicine, University of Minnesota Medical School; • Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments

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