SDS PAGE part 2 Details

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=GRZ48RGY59E

For more information, log on to- • http://shomusbiology.weebly.com/ • Download the study materials here- • http://shomusbiology.weebly.com/bio-m... • SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry, forensics, genetics, molecular biology and biotechnology to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. In most proteins, the binding of SDS to the polypeptide chain imparts an even distribution of charge per unit mass, thereby resulting in a fractionation by approximate size during electrophoresis. Proteins that have a greater hydrophobic content, for instance many membrane proteins, and those which are in their native environment interacting with surfactants, are intrinsically harder to treat accurately using this method, due to the greater variability in the ratio of bound SDS. Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia. • Link- http://en.wikipedia.org/wiki/Main_Page

#############################

New on site