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Bouins fluid Genders Fluid Cytological Fixatives Nuclear Fixatives Histopathology
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Bouin's fluid • a. Saturated aqueous picric acid 75 ml • b. Formalin 25 ml • c. Glacial acetic acid 5 ml • Specific features • Penetrates rapidly and evenly and causes little shrinkage. • .Excellent fixative for testicular and intestinal biopsies because it gives very good • nuclear details, is used for oligospermia and infertility studies. • Good fixative for glycogen. • Itis necessary to remove excess picric acid by alcohol treatment. • 13. Gender's Fluid - It is better fixative for glycogen. • a. Saturated picric acid in 95% v/v/ alcohol - 80ml • b. Formalin 15ml • c. Glacialacetic acid 5ml • Cytological Fixatives • Cytological fixatives are sub divided into two parts: • A. Nuclear fixatives • B. Cytoplasmic fixatives • Nuclear Fixatives • As the name suggests it gives good nuclear fixation. This group includes: • 1. Carnoy's Fluid. 60 ml • a. Absolute alcohol • b. Chloroform 30 ml • c. Glacialacetic acid 10ml • Specific features • It penetrates very rapidly and gives excellent nuclear fixation. • Good fixative for carbohydrates. • Nissil substance and glycogen are preserved. • • #dpmitg.com #dpmiindia.com #DMLT #DPMI #Histopathology
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