How to Create DendrogramsPhylogenetic Trees in PFGESDSPAGEWestern Blot

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=O6vpNnuN_OQ

In this video I'll be showing you how to visualise the similarities between lanes taken from different gels or blots in the form of dendrogams or similarity tables. • 00:00 - Intro • 00:16 - Selecting Lane Set to Convert to Dendrogram • 00:33 - Band Matching Before Dendrogram Creation • 00:48 - Clustering Screen Description • 01:07 - Blank Clustering Screen • 01:17 - Algorithms for Data Generation • 01:23 - Create Your Dendrogram • 01:38 - Create Your Similarity Tables • 02:28 - Explanation of Cophenetic Correlation and Similarity Scores • CLIQS 1D Pro allows the building of a database of lanes and bands extracted from images of 1D gels and blots which can then be searched and compared to unknown samples to quickly identify them. The algorithms embedded within 1D Pro effectively compensate for any distortions between different gel runs to allow accurate comparisons and matching between samples as if they were all run on the same gel at the same time. • CLIQS 1D Pro was specifically designed to facilitate research techniques such as Inter simple sequence repeat (ISSR)-PCR, PFGE bacterial DNA fingerprinting (PFGE), denaturing gradient gel electrophoresis (DDGE), restriction fragment length polymorphism, enterobacterial repetitive Intergenic Consensus (ERIC) PCR and single-strand conformation polymorphism (SSCP) electrophoresis. • For a free trial of CLIQS 1D Pro to see how well it works creating a database from your own images, please fill in the form here: https://totallab.com/products/cliqs-1... • See our other image analysis-based products here: https://totallab.com/products/ • And if you want to get in touch with us here at TotalLab: • Website: http://www.totallab.com/ • LinkedIn: / totallab-ltd • #blot #electrophoresis #dnafingerprinting #CLIQS #CLIQS1DPro #qualitycontrol #DNAgel #ISSR #PFGE #DGGE #RFLP #ERICpcr #SSCP #ISSR #dendrogram

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