Bulk RNASeq Data Analysis Mapping with HiSat2 and Quantification with HTSeq Output Overview

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=PQebW8pv280

In this tutorial, you will: • Learn about the various outputs that we obtain after running an RNA-Seq pipeline on the T-BioInfo Platform (https://server.t-bio.info/). • Pipeline Workflow: Start → Trimmomatic → PCR Clean → HiSat2 → HTseq → End. • Outputs obtained range from mapping stats tables to several gene expression files whose counts of expression can be in FPKM, read counts, or TPM (normalized expression). • Reference Resources Used In Video Tutorial: • Course 5: Transcriptomics - https://learn.omicslogic.com/courses/... • Patient Derived Xenograft Models - https://learn.omicslogic.com/courses/... • T-BioInfo Platform - https://server.t-bio.info/ • ABOUT OUR CHANNEL • Our channel is about bioinformatics and its application to various biomedical and biotechnology challenges. We cover lots of cool topics on data analysis, machine learning and various technologies that generate high-throughput data like genomics, transcriptomics, metagenomics, epigenomics and others. • Check out our channel here: • / pine-biotech • Don’t forget to subscribe! • All of this content and more can be found on our training portal: • https://learn.omicslogic.com • CHECK OUT OUR OTHER VIDEOS • Getting Started with Transcriptomics Data Analysis: Bulk RNA Seq Data Analysis - processing raw sequencing data with Bowtie2 and generating a table of expression with RSEM - • Bulk RNA Seq Data Analysis: Mapping w... • Transcriptomics Data Analysis: Processing FASTQ files using Trimmomatic, Bowtie2-t, RSEM, Review of Output Files (Bulk RNA-Seq Analysis) - • Bulk RNA-Seq Analysis: Processing FAS... • Analysis of Bulk RNA-Seq Data: Mapping Reads on Genome using HiSat2 and quantifying gene expression with HTseq (Setting up the Pipeline on T-BioInfo) - • Bulk RNA-Seq Data Analysis: Mapping w... • Differential Gene Expression: Analysis of Bulk RNA Seq with DESEQ2 on the T-BioInfo Platform (Getting started) - • Differential Gene Expression: Bulk RN... • Differential Gene Expression: Bulk RNA Seq Analysis Results (DESEQ2 Output Review) - • Differential Gene Expression: Bulk RN... • Differential Gene Expression Analysis: Gene-set Enrichment Analysis (GSEA) - • Differential Gene Expression Analysis... • Differential Gene Expression Gene Enrichment Analysis (Output Review) - • Differential Gene Expression Gene Enr... • We offer bioinformatics training and analysis software, check them out here: • https://learn.omicslogic.com • https://server.t-bio.info • FIND US AT • https://pine-biotech.com • GET IN TOUCH • Contact us on marketing at omicslogic.com • FOLLOW US ON SOCIAL • Get updates or reach out to Get updates on our Social Media Profiles! • Twitter: / pinebiotech • Facebook: / pinebiotech • Instagram: / bioinformaticspine • LinkedIn: / omicslogic

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