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Probe-based qPCR allows for specific hybridization. The targeted nature of probe-based qPCR leads to low background, or nonspecific fluorescence, and eliminates the presence of false positives. You can also label probes with different, distinguishable reporter dyes to allow for amplification of two distinct sequences in one reaction tube. • To learn more, visit https://www.sigmaaldrich.com/video/li...
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